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PubMed · 6793550

Rapid screening method for aflatoxin M1 in milk.

C E Holaday
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Abstract

A rapid screening method for detecting aflatoxin M1 in milk has been developed, based on minicolumn chromatography and requiring 8-10 min for each test. The minicolumn is packed with dry Florisil (100-200 mesh) on the bottom, anhydrous Na2SO4 as the next layer, topped with neutral alumina (70-200 mesh) to which 8% water (wet basis) has been added. A blue fluorescent band at the Florisil-Na2SO4 interface indicates the presence of aflatoxin M1. The limit of detection is estimated to be about 0.2 microgram/kb. Because several items are disposable, both the time to maintain glassware and the cost per determination are reduced.

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Aflatoxin M1AflatoxinsAnimalsCattleChromatographyChromatography, High Pressure LiquidChromatography, Thin LayerMilkJournal Article

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C E Holaday. 1981. Rapid screening method for aflatoxin M1 in milk.. https://pubmed.ncbi.nlm.nih.gov/6793550/

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