A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein.

E M Salonen

doi:10.1016/0022-1759(82)90208-3

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A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein.

E M Salonen

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Abstract

C-reactive protein (CRP) is an acute-phase reactant the concentration of which increases significantly following tissue injury or inflammation. I report here the development of a rapid and sensitive solid-phase enzyme immunoassay (EIA) for determination of serum CRP. The inclusion of 4% (w/v) polyethylene glycol (PEG) in the diluent buffers made it possible to quantitate CRP within 4 h at room temperature. The minimum detectable amount was about 500 pg CRP/ml. Excellent correlation in determination of serum CRP was found between solid-phase EIA and nephelometric assay, radial immunodiffusion and the qualitative latex agglutination tests.

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Animals C-Reactive Protein Humans Immunodiffusion Immunoenzyme Techniques Latex Fixation Tests Nephelometry and Turbidimetry Rabbits Swine Journal Article Research Support, Non-U.S. Gov't

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E M Salonen. 1982. A rapid and sensitive solid-phase enzyme immunoassay for C-reactive protein.. https://doi.org/10.1016/0022-1759(82)90208-3

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