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PubMed · 6804081

A human plasma cell line: induction and characterization.

C H Miller·A Carbonell·R Peng·T Paglieroni·M R MacKenzie
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Abstract

A stable line of IgG K producing human plasma cells was established from a myelomatous human bone marrow using conditioned media from a rapidly metabolizing lymphoblast line, RPMI 4098. Growth in RPMI 1640 (15% fetal calf serum) at 6% CO2 promoted a 62-hour doubling time with a preferred cell concentration of 1 x 10(6)/mL. Surface marker studies showed: no receptors for sheep erythrocytes, no surface immunoglobulins, variable number of cells bearing complement receptors and 83% bearing Fc receptors. Although transmission electron micrographs demonstrated a poorly developed endoplasmic reticulum, radioimmunoassay showed 23 ng IgG and 28.7 ng Kappa were produced by 1 x 10(6) cells in 72 hours. Further, the cells are lipase, esterase and Epstein-Barr nuclear antigen negative. ASG banding showed a total chromosome number that varied from 46--49. Since the number of human plasma cell lines is limited, it is felt that this line will augment the immunobiological study of human myeloma.

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Bone MarrowCell DivisionCell LineCell MembraneChromosome BandingEndoplasmic ReticulumHumansImmunoglobulin GImmunoglobulin kappa-ChainsMultiple MyelomaPlasma CellsPlasmacytomaReceptors, ComplementReceptors, FcJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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C H Miller, A Carbonell, R Peng, T Paglieroni, M R MacKenzie. 1982-05-15. A human plasma cell line: induction and characterization.. https://doi.org/10.1002/1097-0142(19820515)49%3A10%3C2091%3A%3Aaid-cncr2820491021%3E3.0.co%3B2-9

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