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PubMed · 6807263

The acidic macromolecules in rabbit cortical bone tissue.

A G Diamond·J T Triffitt·G M Herring
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Abstract

Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.

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AnimalsBone and BonesChondroitin SulfatesChromatographyEdetic AcidElectrophoresis, Polyacrylamide GelGlycosaminoglycansKeratan SulfateMacromolecular SubstancesProteoglycansRabbitsSialoglycoproteinsJournal Article

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A G Diamond, J T Triffitt, G M Herring. 1982. The acidic macromolecules in rabbit cortical bone tissue.. https://doi.org/10.1016/0003-9969(82)90164-9

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