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A solid-phase immunofluorescence assay (SIFA) using membrane filters.

B Micheel·U Karsten·H Fiebach
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Abstract

A solid-phase immunofluorescence assay (SIFA) is presented which allows the demonstration of antibodies against soluble antigens. The test is performed by successively incubating nitrate cellulose discs in microtitration plates with anti-Ig, the sample to be tested and FITC-labeled antigen. The fluorescence intensity of the discs is then measured by using a microfluorometer. The assay is simple, needs a small amount of material and allows to test a large number of samples.

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AnimalsAntibodiesAntigen-Antibody ReactionsAntigensCattleFluorescent Antibody TechniqueGoatsHumansHybridomasMiceSerum AlbuminSheepJournal Article

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B Micheel, U Karsten, H Fiebach. 1982. A solid-phase immunofluorescence assay (SIFA) using membrane filters.. https://doi.org/10.1016/s0065-1281(82)80005-6

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