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Light-chain diversification during pre-B cell differentiation.

L Lafleur·G Mercier·V V Micusan
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Abstract

The possibility that a committed normal pre-B cell can generate progeny expressing more than one light chain was studied by isoelectric focusing of supernatants from pre-B cells cultured at limiting dilution. Supernatants from mitogen-stimulated mature spleen B-cells analysed on day 6 have shown the presence of a rather homogeneous IgM spectrotype profile. Supernatants from the 8-10-day cultures were usually negative. Isoelectric focusing profiles of supernatants from cultures of bone marrow pre-B cells were different from those of mature spleen cells. Many IgM spectrotypes appeared in the culture supernatants of bone marrow pre-B cells between days 6 and 13, whether the cultures were negative or restricted in their IgM profiles on day 6. These results support the idea that normal pre-B cells, during differentiation, can associate a variety of light chains with an already chosen mu heavy chain.

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AnimalsB-LymphocytesBone MarrowBone Marrow CellsCell DifferentiationCells, CulturedFemaleImmunoglobulin Light ChainsImmunoglobulin MIsoelectric FocusingMaleMiceMice, Inbred BALB CRatsRats, Inbred StrainsJournal ArticleResearch Support, Non-U.S. Gov't

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L Lafleur, G Mercier, V V Micusan. 1982. Light-chain diversification during pre-B cell differentiation.. https://doi.org/10.1016/0161-5890(82)90268-1

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