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Solid-phase vitamin B12 assays using polyacrylamide-bound intrinsic factor and polyacrylamide-bound R-binder.

M Muir·I Chanarin
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Abstract

A new solid phase vitamin B12 assay is described using intrinsic factor to measure microbiologically-available B12 and R-binder to measure total B12. The solid phase reagent consists of intrinsic factor coupled to polyacrylamide beads and salivary R-binder coupled to polyacrylamide beads. The assay is simple to perform and separates completely sera from controls and patients with megaloblastic anaemia due to B12 deficiency.

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Acrylic ResinsAnemia, MegaloblasticHumansHydrogen-Ion ConcentrationIntrinsic FactorLightMethodsNuclear ProteinsNucleoproteinsOsmolar ConcentrationTime FactorsVitamin B 12Vitamin B 12 DeficiencyPolyacrylamidesJournal Article

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BibTeXRIS

M Muir, I Chanarin. 1983. Solid-phase vitamin B12 assays using polyacrylamide-bound intrinsic factor and polyacrylamide-bound R-binder.. https://doi.org/10.1111/j.1365-2141.1983.tb02043.x

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