Studies on the degranulation test for carcinogens.

T W Poole; D V Parke

doi:10.1016/0014-5793(83)80335-4

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Studies on the degranulation test for carcinogens.

T W Poole·D V Parke

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Abstract

The radiometric assay of degranulation of the hepatic endoplasmic reticulum by chemical carcinogens has been re-examined. Both 1,2,3,4,- and 1,25,6-dibenzanthracenes caused degranulation of rough membranes in vitro; with acetamidofluorenes and naphthylamines the carcinogenic analogues caused moderately greater degranulation. Degranulation by 1,2,3,4-dibenzantracene was rapid and was maximal after 5 min incubation. Pretreatment of animals with phenobarbital or methylcholanthrene increased the fraction of rough membranes, but these were not fully granulated. The assay is limited in specificity and sensitivity because the 1.35 M sucrose gradient does not effectively separate rough and smooth membranes, and sedimented membranes are contaminated with aggregates of free ribosomes.

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1-Naphthylamine 2-Acetylaminofluorene 2-Naphthylamine Animals Benz(a)Anthracenes Biological Assay Carcinogens Cell Fractionation Dimethyl Sulfoxide Endoplasmic Reticulum Fluorenes Liver Male Rats Rats, Inbred Strains Journal Article Research Support, Non-U.S. Gov't

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T W Poole, D V Parke. 1983-01-10. Studies on the degranulation test for carcinogens.. https://doi.org/10.1016/0014-5793(83)80335-4

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