Human serum low density lipoprotein-sodium deoxycholate interaction.

J Q Oeswein; P W Chun

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PubMed · 6833223

Human serum low density lipoprotein-sodium deoxycholate interaction.

J Q Oeswein·P W Chun

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Abstract

The binding of sodium deoxycholate to low density lipoprotein (LDL) is a relatively fast process, as compared to the lipid displacement reaction and solubilization. The initial reaction has a bimolecular rate constant of approximately 539 M-1 S-1. In the presence of 1 mM sodium deoxycholate, below critical micelle concentration, 0.04 g of sodium deoxycholate was bound per g of LDL, resulting in an increase in particle radius from 102 to 128 +/- 0.3 A and an axial ratio of 5.6 for a prolate ellipsoid. Such increases were also observed using higher sodium deoxycholate concentrations and are apparently due to sodium deoxycholate-induced elongation or distortion of certain portions of LDL.

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Chromatography, Gel Deoxycholic Acid Humans Kinetics Lipoproteins, LDL Mathematics Molecular Weight Protein Binding Spectrophotometry Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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J Q Oeswein, P W Chun. 1983-03-25. Human serum low density lipoprotein-sodium deoxycholate interaction.. https://pubmed.ncbi.nlm.nih.gov/6833223/

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