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A simple, rapid, high-resolution chromosome technic for lymphocytes.

Y S Kao·J Whang-Peng·E Lee
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Abstract

Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.

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HumansKaryotypingLymphocyte ActivationLymphocytesPhytohemagglutininsProphaseJournal Article

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Y S Kao, J Whang-Peng, E Lee. 1983. A simple, rapid, high-resolution chromosome technic for lymphocytes.. https://doi.org/10.1093/ajcp%2F79.4.481

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