A simple micromethod for leukocyte rosetting without cell purification.

E S Buescher; J I Gallin

doi:10.1016/0022-1759(83)90155-2

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PubMed · 6863943

A simple micromethod for leukocyte rosetting without cell purification.

E S Buescher·J I Gallin

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Abstract

Conventional methods for leukocyte rosetting suffer from 2 major drawbacks: the length of time required for leukocyte purification prior to rosetting, and the necessity for large volumes of blood from which to purify the leukocytes. A simple method for leukocyte rosetting is described which uses small quantities (less than 100 microliter) of heparinized whole blood, does not require prior leukocyte purification and can be completed within 1 h of blood drawing. Percentages of rosetting polymorphonuclear leukocytes in cell preparations obtained by standard purification methods showed a close correlation (P less than 0.001) with percentages obtained by the method described. Additionally, the method described can potentially be applied to other non-neutrophil rosetting leukocytes in blood or body fluids.

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E S Buescher, J I Gallin. 1983-07-15. A simple micromethod for leukocyte rosetting without cell purification.. https://doi.org/10.1016/0022-1759(83)90155-2

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