Decrease in the levels of specific non-polysomal messenger ribonucleoproteins during the mouse sarcoma-180 ascites cell cycle.

T E Geoghegan; S J Knoop; C Chitpatima

doi:10.1016/0006-291x(83)91087-2

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Decrease in the levels of specific non-polysomal messenger ribonucleoproteins during the mouse sarcoma-180 ascites cell cycle.

T E Geoghegan·S J Knoop·C Chitpatima

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Abstract

A few mRNAs in S-180 ascites cells are found as non-polysomal messenger ribonucleoprotein particles, whose translation is restricted both in vivo and in vitro. To determine if these mRNPs remain untranslated throughout the cell cycle we obtained S-180 ascites cells enriched in cell cycle stages by centrifugation through ficoll gradients. The "inactive" mRNA species were clearly present in the pre-polysomal fraction from S and pre-S phase cells, however they were not detected in the mRNP fraction from post S phase cells. Hybridization of a cloned cDNA probe for one of these species (P-40) to polysomal RNA from pre- or post-S phase cells demonstrated it was present in substantially higher concentrations in polysomes from post-S phase cells, consistent with its translation after S phase. We also observed that a poly (A)-minus form of the mRNA coding for actin was restricted to post S phase cells.

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Animals Ascitic Fluid Cell Cycle Cell-Free System In Vitro Techniques Mice Nucleoproteins Polyribosomes Protein Biosynthesis RNA, Messenger RNA, Neoplasm Ribonucleoproteins Sarcoma 180 Journal Article Research Support, Non-U.S. Gov't

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T E Geoghegan, S J Knoop, C Chitpatima. 1983-06-29. Decrease in the levels of specific non-polysomal messenger ribonucleoproteins during the mouse sarcoma-180 ascites cell cycle.. https://doi.org/10.1016/0006-291x(83)91087-2

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