Photoaffinity labeling: theory and practice.

R J Guillory; S J Jeng

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PubMed · 6873311

Photoaffinity labeling: theory and practice.

R J Guillory·S J Jeng

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Abstract

The potential for generating a reactive reagent in situ by photoexcitation and for labeling sites not containing reactive nucleophilic residues provides photoaffinity labeling with a special advantage in the study of ligand-binding sites. There are, however, problems in the application of the photoaffinity technique. A number of concerns about this approach are examined by using as a background studies with arylazido-beta-alanyl nucleotide probes developed in this laboratory. It is concluded that photoaffinity labeling is likely to emerge as one of the major techniques for elucidating structure-function relationships within biological membranes. In the future the design of photoprobes with shorter activation half-lives may make it possible to investigate time-dependent (i.e., conformational) changes occurring at the active site of enzymes and within complex ligand-binding sites.

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Adenosine Triphosphate Affinity Labels Azides Photochemistry Structure-Activity Relationship Journal Article

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R J Guillory, S J Jeng. 1983. Photoaffinity labeling: theory and practice.. https://pubmed.ncbi.nlm.nih.gov/6873311/

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