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An 'affinity' method for preparing polypeptides enriched in the collagen-associated Ehrlich chromogen.

J E Scott·E W Hughes·A Shuttleworth
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Abstract

The collagen-associated compound which reacts at room temperature with acid dimethylaminobenzaldehyde (Ehrlichs reagent) also reacts in acid with aryl-diazonium reagents. We have developed a convenient method for isolating polypeptides associated with the Ehrlich chromogen from collagen digests utilizing diazotized arylamino-cellulose supports. These peptides, in which the Ehrlich chromogen is 'labeled' with yellow diazo color, constitute less than 0.5% of the collagen, and have amino acid patterns similar to those around the cross link regions. The chromogen is not identical with pyridinoline, also thought to be a polyvalent cross link.

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AnimalsAzo CompoundsBenzaldehydesCattleCelluloseChromogenic CompoundsCollagenPeptidesJournal Article

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J E Scott, E W Hughes, A Shuttleworth. 1983. An 'affinity' method for preparing polypeptides enriched in the collagen-associated Ehrlich chromogen.. https://doi.org/10.1093/jb%2F93.3.921

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