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Decrease in apparent Km for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes.

S W Edwards·M B Hallett·D Lloyd·A K Campbell
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Abstract

The respiratory burst of polymorphonuclear leukocytes, induced by the addition of chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine) and cytochalasin B was found to consist of two phases. The first phase of very rapid oxygen uptake lasted 1-3 min. and was followed by a second more prolonged phase of lower magnitude. The apparent Km for oxygen of unstimulated cells was 9.6 +/- 0.67 microM, while that of the second phase of stimulation was 3.7 +/- 1.6 microM oxygen. The possibility that lowered oxygen concentrations may regulate polymorphonuclear leukocyte activity in some pathological conditions is discussed.

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AnimalsChemotaxis, LeukocyteCytochalasin BKineticsN-FormylmethionineN-Formylmethionine Leucyl-PhenylalanineNeutrophilsOligopeptidesOxygenOxygen ConsumptionRatsJournal ArticleResearch Support, Non-U.S. Gov't

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S W Edwards, M B Hallett, D Lloyd, A K Campbell. 1983-09-05. Decrease in apparent Km for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes.. https://doi.org/10.1016/0014-5793(83)80730-3

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