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A convenient method for the determination of non-specific binding in commercially available solid phase iodine labeled radioimmunoassay kits.

P Carter
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Abstract

57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range of the calibrated standard curve. In the present state of technology correction appears to have a trifling effect on the overall accuracy of the assay; however, the method as described allows the laboratorian to confirm for himself when testing a new product.

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Cobalt RadioisotopesDigoxinEvaluation Studies as TopicHumansHydrocortisoneIodine RadioisotopesRadioimmunoassayReagent Kits, DiagnosticComparative StudyJournal Article

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BibTeXRIS

P Carter. 1978. A convenient method for the determination of non-specific binding in commercially available solid phase iodine labeled radioimmunoassay kits.. https://doi.org/10.1016/s0009-9120(78)90070-x

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