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PubMed · 6892923

A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.

T P Shearer·L G Hunsicker
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Abstract

A rapid method for embedding tissues for electron microscopy is described. This method, which can be completed within 5 hr, uses 1,4-dioxane as the final dehydrating agent and Polybed 812 as the embedding medium. Satisfactory preservation of cellular structures is consistently achieved with a variety of normal and diseased tissues. This method may be of particular value to diagnostic electron microscopy laboratories where time and simplicity are critical.

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AnimalsDioxanesDioxinsMicroscopy, ElectronMonocytesNephritisPolymersRabbitsRatsSerum SicknessSpleenJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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T P Shearer, L G Hunsicker. 1980. A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.. https://doi.org/10.1177/28.5.6892923

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