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PubMed · 68958

Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.

C A Marotta·J T Wilson·B G Forget·S M Weissman
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Abstract

Sequences of human beta-globin mRNA were determined by analysis of complementary DNA. beta-mRNA was transcribed into double-stranded cDNA by RNA-dependent DNA polymerase. cDNA was cut by restriction endonucleases and the fragments were terminally labeled by means of polynucleotide kinase and [gamma-32P]ATP. After purification, fragments were degraded by snake venom phosphodiesterase. Alternatively single-stranded [32P]cDNA was prepared by transcription in the presence of [alpha-32P]dCTP and actinomycin D; the product was digested by endonuclease IV and degraded by snake venom phosphodiesterase. cDNA tracts obtained by both labeling methods enabled us to construct a sequence for the translated and 3'-terminal untranslated regions of human beta-mRNA.

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Anemia, Sickle CellBase SequenceDNADNA Restriction EnzymesDactinomycinDeoxyribonucleasesEndonucleasesGlobinsHumansOligodeoxyribonucleotidesPhosphoric Diester HydrolasesPolynucleotide 5'-Hydroxyl-KinaseProtein BiosynthesisRNA, MessengerRNA-Directed DNA PolymeraseThalassemiaTranscription, GeneticJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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C A Marotta, J T Wilson, B G Forget, S M Weissman. 1977-07-25. Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.. https://pubmed.ncbi.nlm.nih.gov/68958/

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