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PubMed · 69325

Human gene mapping using an X/autosome translocation.

E Solomon·M Bobrow·P N Goodfellow·W F Bodmer·D M Swallow·S Povey·B Noël
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Abstract

Human fibroblasts containing a translocation between the X chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, IR. Resulting hybrids, selected in HAT medium, retained the X/15 chromosome. Hybrids which were counterselected in 6-thioguanine lost this chromosome. The X-linked markers glucose-6-phosphate dehydrogenase (G6PD), phosphoglycerate kinase (PGK), and hypoxanthine phosphoribosyl transferase (HPRT), and the non-X-linked markers pyruvate kinase (PKM2) mannose phosphate isomerase (MPI), N-acetyl hexosaminidase A (HEXA) and beta2-microglubulin (beta2-m) all segregated in concordance with the X/15 translocation chromosome. The latter markers have been assigned to chromosome 15. Selection against the X/15 chromosome was done using antihuman beta2-m serum. Electrophoretic and immunochemical analyses of the N-acetyl hexosaminidases A and B in these hybrids were performed.

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AcetylglucosaminidaseBeta-GlobulinsCarbohydrate EpimerasesChromosome MappingChromosomes, Human, 13-15GenesGenetic LinkageHexosaminidasesHumansHybrid CellsKaryotypingMannosePyruvate KinaseSex ChromosomesSugar PhosphatesTranslocation, Geneticbeta 2-MicroglobulinJournal Article

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E Solomon, M Bobrow, P N Goodfellow, W F Bodmer, D M Swallow, S Povey, B Noël. 1976. Human gene mapping using an X/autosome translocation.. https://doi.org/10.1007/bf01542626

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