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A silver impregnation method for nervous tissue suitable for routine use with mounted sections.

J M Loots·G P Loots·W S Joubert
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Abstract

A simple, reliable silver impregnation method for nervous tissue is described for tissues fixed in various fixatives including formalin, Bouin, and Susa. Sections are impregnated in a solution containing 1 g Protargol, 2 ml of a 1% Cu(NO3)2 solution, 2 ml of a 1% AgNO3 solution, and 2-4 drops 30% H2O2 in 100 ml distilled water. Sections are impregnated 2-5 days at 37 C and thereafter reduced in a hydroquinone-formalin solution. This is followed by gold toning and subsequent reduction, dehydration and mounting. This method has been found to be very reliable and selective.

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AnimalsAxonsCatsChickensCollagenFixativesHumansNerve TissueNeurofibrilsRabbitsRatsSilverStaining and LabelingJournal Article

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J M Loots, G P Loots, W S Joubert. 1977. A silver impregnation method for nervous tissue suitable for routine use with mounted sections.. https://doi.org/10.3109/10520297709116752

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