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Modified tannin-osmium conductive staining method for non-coated scanning electron microscope specimens. Its application to microdissection scanning electron microscopy of the spleen.

T Murakami·K Vamamoto·T Itoshima·S Irino
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Abstract

Supplement of guanidine hydrochloride to the original tannic acid solution of MURAKAMI (1973) eliminated, without debilitating the conductivity effect, the vexatious treatment with different amino-acids and sucrose in the previous method (MURAKAMI, 1974). The procedures and advantages of this simplified modification are described using human spleen samples; attempt was made to elucidate the structure of arterial terminals by dissecting the specimens under the scanning electron microscope.

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DissectionHumansHydrolyzable TanninsMicroscopy, Electron, ScanningSpleenStaining and LabelingJournal Article

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T Murakami, K Vamamoto, T Itoshima, S Irino. 1977. Modified tannin-osmium conductive staining method for non-coated scanning electron microscope specimens. Its application to microdissection scanning electron microscopy of the spleen.. https://doi.org/10.1679/aohc1950.40.35

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