PubMed · 6969424
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C Janeway, B Jones, H Binz, H Frischknecht, H Wigzell. 1980. T-Cell receptor idiotypes.. https://doi.org/10.1111/j.1365-3083.1980.tb00044.x
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Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative functional homology with CHO1, the overexpression of the PEL1 gene in the cho1 null mutant did not restore the wild-type properties of the transformed cells and failed to stimulate the incorporation of L-[3-3H]serine into total lipids of the intact yeast cells.
An immunoblotting method is described that permits sequential detection of multiple antigens on a single protein blot without stripping off prior antibodies. The procedure utilizes horseradish peroxidase (HRPase)-based detection with a chemiluminescent substrate. After detection by enhanced chemiluminescence (ECL) with exposure to X-ray film, the antigen-antibody complexes on the blot are reacted with a chromogenic substrate (either 3.3'-diaminobenzidine [DAB] or SG [Vector Labs, Inc.]) which renders the antigen-antibody-HRPase complexes unreactive in subsequent reprobings of the same membrane with additional antibodies using the same detection method. Because no stripping is involved, immobilized proteins are not lost from the membrane, thus allowing for multiple sequential reprobings of the same membrane with different primary antibodies (> or = 12) and retention of strong signal intensities for all antibody probings. A variation of the method (the "rainbow Western") is described in which four different HRPase-colorimetric substrates that produce black, brown, red, and green colors are employed sequentially for detection and simultaneous display of four different antigens on the same blot. Both techniques could be particularly valuable for analysis of cellular populations that are difficult to isolate in large numbers or of clinical specimens where the amounts of protein samples that can be obtained are limiting or only available on a one-time basis.
alpha beta T cells specifically recognize a ligand composed of a peptide bound to a self-major-histocompatibility-complex molecule, but the recognition of slightly altered ligands by T cells can lead to a partial activation. This flexibility is crucial for T-cell development and can have both beneficial and harmful effects on peripheral T cells.