Phloretin sensitive active urea absorption in frog skin.

M Svelto; V Casavola; G Valenti; C Lippe

doi:10.1007/bf00589096

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Phloretin sensitive active urea absorption in frog skin.

M Svelto·V Casavola·G Valenti·C Lippe

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Abstract

This report presents evidence for urea active absorption by isolated skin of Rana esculenta. One of the supporting factors of such evidence is that at a low concentration the urea influx is five times greater than the outflux, in the absence of a chemical gradient. The transport shows a saturation kinetics with an apparent Km = 1.33 mM and is inhibited by un uncoupling agent (FCCP). 5 x 10(-4) M Phloretin, added to the external side, markedly inhibits inward urea transport, whereas it is ineffective when added to the serosal fluid. This provides evidence for a phloretin-sensitive mechanism located at the external side of the epithelium. Phloretin stimulates the sodium active transport; the possible coupling of urea and sodium movement is analysed.

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Animals Biological Transport, Active In Vitro Techniques Phloretin Rana esculenta Skin Absorption Sodium Urea Journal Article

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M Svelto, V Casavola, G Valenti, C Lippe. 1982. Phloretin sensitive active urea absorption in frog skin.. https://doi.org/10.1007/bf00589096

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