Improved reaction buffers for solid-phase enzyme immunoassay without interference by serum factors.

K Kato; Y Umeda; F Suzuki; A Kosaka

doi:10.1016/0009-8981(80)90043-1

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Improved reaction buffers for solid-phase enzyme immunoassay without interference by serum factors.

K Kato·Y Umeda·F Suzuki·A Kosaka

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Abstract

A gelatin moderately digested by a protease, was as effective as original gelatin to remove the interference by serum factors with a sandwich immunoassay for insulin. Digestion of gelatin resulted in decrease in the viscosity, and the buffer containing the digested gelatin did not show gelation at 4 degrees C. Therefore the buffer involving the digested gelatin might be widely useful for the immunoassay without interference by serum factors.

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Buffers Cold Temperature Gelatin Humans Immunoenzyme Techniques Insulin Peptide Hydrolases Viscosity Journal Article

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K Kato, Y Umeda, F Suzuki, A Kosaka. 1980-03-28. Improved reaction buffers for solid-phase enzyme immunoassay without interference by serum factors.. https://doi.org/10.1016/0009-8981(80)90043-1

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