Evaluating optical filter efficiency in a flow cytometer.

M R Loken

doi:10.1177/28.10.6999085

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PubMed · 6999085

Evaluating optical filter efficiency in a flow cytometer.

M R Loken

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Abstract

A simple yet effective method of testing optical filters under normal operating conditions on a Fluorescence Activated Cell Sorter is described. The spectral characteristics of three filters are compared to demonstrate the methods of determining which filter is the best in a particular situation. These tests include determining the efficiency of transmitting fluorescent light, determining the efficiency of removing scattered light, and detecting fluorescence originating from the filter itself.

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Animals Chickens Cytological Techniques Filtration Spectrometry, Fluorescence Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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M R Loken. 1980. Evaluating optical filter efficiency in a flow cytometer.. https://doi.org/10.1177/28.10.6999085

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