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PubMed · 7009163

Flow microfluorometric analysis of alloantigen expression during T cell development.

T A Potter·P M Hogarth·I F McKenzie
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Abstract

The fluorescence-activated cell sorter (FACS) was used to examine the expression of several alloantigens on T cells: Thy-1, Lyt-1.1, Lyt-2.1, Ly-5, Ly-6 and Ly-7. Antibodies secreted by monoclonal cell lines were used to characterize the Thy-1:2, Lyt-1.1 and Lyt-2.1 antigenic determinants, whereas Ly-5.1, Ly-6.2 and Ly-7.2 determinants were identified with alloantisera raised by conventional hyperimmunization. Using indirect immunofluorescence with fluorescein isothiocyanate-conjugated reagents, the profiles obtained with the FACS demonstrated that: (a) the expression of Thy-1 is greater on normal thymocytes than on cortisone-resistant thymocytes (CRT), lymph node and spleen cells; (b) in contrast to Thy-1, the expression of Ly-1 on normal thymocytes is less than on CRT, lymph node and spleen cells; (c) about 90% of normal thymocytes abut less than 50% of CRT, spleen and lympho node T cells are Lyt-2+ and (d) although weakly expressed on normal thymocytes, the amount of Ly-6 and Ly-7 present on peripheral T cells was considerably increased. The significance of these findings and the potential for further analysis of T cell developmental pathways is discussed.

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AnimalsCell CountCell DifferentiationCortisoneImmune SeraImmunologic TechniquesIsoantigensLymph NodesMiceMice, Inbred BALB CMice, Inbred C3HRabbitsSpleenT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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T A Potter, P M Hogarth, I F McKenzie. 1980. Flow microfluorometric analysis of alloantigen expression during T cell development.. https://doi.org/10.1002/eji.1830101202

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