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Improved streptococcal grouping antisera containing polyethylene glycol.

J R George·H Ashworth·R R Facklam·W K Harrell·D F Palmer
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Abstract

Antisera to streptococcal groups A through G containing 4% polyethylene glycol 6000 were prepared and evaluated. Seventy strains of homologous and heterologous beta- and non-beta-hemolytic streptococci were included in the evaluation. Homologous reactions were determined against extracts prepared by four extraction methods: hot hydrochloric acid (Lancefield) extraction, autoclave extraction, hot formamide extraction, and nitrous acid extraction. Enhancement of the precipitin reaction in the presence of polyethylene glycol 6000 permitted a fourfold dilution of all antisera for use in the capillary precipitin test. At this dilution, the potency of the antisera exceeded requirements established for these reagents.

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Cross ReactionsImmune SeraPolyethylene GlycolsPrecipitin TestsSerotypingStreptococcusStreptococcus agalactiaeStreptococcus pyogenesJournal Article

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J R George, H Ashworth, R R Facklam, W K Harrell, D F Palmer. 1981. Improved streptococcal grouping antisera containing polyethylene glycol.. https://doi.org/10.1128/jcm.14.4.433-436.1981

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