DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system.

H M Kronenberg; B E Roberts; J F Habener; J T Potts; A Rich

doi:10.1038/267804a0

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DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system.

H M Kronenberg·B E Roberts·J F Habener·J T Potts·A Rich

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Abstract

DNA complementary in sequence to the messenger RNA for pre-proparathyroid hormone was synthesised using reverse transcriptase. In a linked transcription-translation system using RNA polymerase and cell-free extract from wheat germ, the DNA directed the synthesis of a protein identified as pre-proparathyroid hormone by N-terminal sequencing and by electrophoretic and immunologic criteria.

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Amino Acid Sequence Animals Base Sequence Cattle Cell-Free System DNA DNA-Directed RNA Polymerases Epitopes Genes Molecular Weight Parathyroid Hormone Protein Biosynthesis Protein Precursors RNA, Messenger Transcription, Genetic Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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H M Kronenberg, B E Roberts, J F Habener, J T Potts, A Rich. 1977-06-30. DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system.. https://doi.org/10.1038/267804a0

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