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PubMed · 7090334

Complement in CPD-stored blood.

J A Schifferli·H Boralessa·H L Howarth·J G Whitwam·A J Rees
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Abstract

We studied complement functions, breakdown of C3 and aggregation of immunoglobulins in whole blood stored in citrate phosphate dextrose at 4 degrees C for up to 3 weeks from 10 normal blood donors. No significant changes in total haemolytic complement (CH50) and alternative pathway haemolytic complement activity were detected. However, the complement-mediated capacity of the sera to solubilise a pre-formed immune precipitate decreased significantly at 2 and 3 weeks (p less than 0.02 at 2 weeks, p less than 0.003 at 3 weeks). No C3 conversion could be detected by immunoelectrophoresis but alteration in C3 was attested by a significant increase in breakdown products of C3 by 1 week (p less than 0.002) and all were above the normal range after 3 weeks (p less than 0.0001). No aggregation of immunoglobulins could be detected using two immune complex assays.

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Antigen-Antibody ComplexBlood PreservationCitratesComplement C3Complement Pathway, AlternativeComplement System ProteinsGlucoseHemolysisHumansJournal ArticleResearch Support, Non-U.S. Gov't

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J A Schifferli, H Boralessa, H L Howarth, J G Whitwam, A J Rees. 1982. Complement in CPD-stored blood.. https://doi.org/10.1111/j.1423-0410.1982.tb01095.x

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