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PubMed · 7095451

Collagen types in keratoconus.

T M Radda·E J Menzel·H Freyler·H D Gnad
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Abstract

Collagen types in normal human and keratoconus corneas were separated by salt fractionation and thermal gelation in the pepsin-soluble fraction of the lyophilized tissues. Peptic digestion indicated no significant differences between normal and keratoconus corneas. Further collagen characterization was performed using SDS-PAGE. Collagen concentration were determined via hydroxyproline. Soluble collagens from normal human cornea represent 85% collagen type I, maximally 10% collagen type III and 5% collagen type V. Soluble collagens of keratoconus corneas consist of 90% type I collagen and maximally 5% type II and type V collagen.

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Chemical FractionationCollagenCorneaElectrophoresis, Polyacrylamide GelFemaleHumansKeratoconusMaleJournal Article

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T M Radda, E J Menzel, H Freyler, H D Gnad. 1982. Collagen types in keratoconus.. https://doi.org/10.1007/bf02175894

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