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PubMed · 7125654

New process for T-2 toxin production.

D Cullen·E B Smalley·R W Caldwell
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Abstract

Strains of Fusarium produced high levels of T-2 toxin when cultured on certain media absorbed into vermiculite. Modified Gregory medium was nutritionally complex (2% soya meal, 0.5% corn steep liquor, 10% glucose) and, when inoculated with the appropriate fungal strain, yielded maximum T-2 toxin within 24 days of incubation at 19 degrees C. On Vogel synthetic medium N (H. J. Vogel, Microb. Genet, Bull. 13:42-43, 1956) supplemented with 5% glucose, optimal toxin levels were synthesized after incubation for 12 to 14 days at 15 degrees C. Fusarium tricinctum T-340 produced 714 and 353 mg/liter on modified Gregory medium and Vogel synthetic medium N plus 5% glucose, respectively. Improved analytical procedures were developed and involved aqueous methanol extraction, purification by liquid-liquid partitions, and gas-chromatographic quantitation.

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Chromatography, GasCulture MediaFusariumGlucoseKineticsSesquiterpenesT-2 ToxinTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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D Cullen, E B Smalley, R W Caldwell. 1982. New process for T-2 toxin production.. https://doi.org/10.1128/aem.44.2.371-375.1982

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