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Platelet aggregation and multiple sclerosis.

I S Neu·M Prosiegel·V Pfaffenrath
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Abstract

Measurements of blood platelet aggregation were carried out in 30 patients suffering from multiple sclerosis (MS) and in 15 healthy individuals. Compared with the control group, the MS patients showed an increase in both spontaneous and induced (ADP and serotonin) platelet aggregation. The possible pathogenetic significance of these results is discussed.

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Adenosine DiphosphateAdolescentAdultFemaleHumansMaleMiddle AgedMultiple SclerosisPlatelet AggregationSerotoninThrombosisJournal Article

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BibTeXRIS

I S Neu, M Prosiegel, V Pfaffenrath. 1982. Platelet aggregation and multiple sclerosis.. https://doi.org/10.1111/j.1600-0404.1982.tb06872.x

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PubMed1997

Monomeric kinesin head domains hydrolyze multiple ATP molecules before release from a microtubule.

Transient kinetic analysis of microtubule-stimulated ATP hydrolysis by the monomeric kinesin motor domain DKH357 was performed to investigate the kinetic pattern of a monomer. Both ATP and ADP produced dissociation of the complex, microtubule (MT).E, of microtubules with DKH357 at a maximum rate of approximately 45 s-1 as determined by decrease in turbidity. The maximum dissociation rate was independent of the KCl concentration between 25 and 200 mM. At subsaturating levels of nucleotide, ATP was more effective than ADP in dissociating DKH357 from MT.E (1.6 and 0.4 microM-1 s-1 for ATP and ADP, respectively, at 50 mM KCl). Addition of ATP to MT.E results in a burst of product formation with a maximum initial rate of approximately 100 s-1 at saturating levels of ATP. This maximum hydrolysis rate of 100 s-1 is similar to the maximum steady state ATPase rate at saturating microtubules of approximately 70 s-1, and thus hydrolysis is at least partially rate-limiting. When the MT lattice was highly occupied with bound DKH357, the amplitude of the burst was approximately 2 per DKH357 active site (superstoichiometric). The rate constant for the burst transient was approximately 45 s-1, which is the same as the rate for dissociation of DKH357 from the microtubule and this suggests that dissociation and termination of the burst phase are coupled. The size of the burst increased with decreasing initial occupancy of the MT lattice with bound DKH357 and approached the value of approximately 4 ATP molecules predicted by previous steady state measurements (Jiang, W., Stock, M., Li, X., and Hackney, D. D., submitted for publication).

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PubMed1997

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