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PubMed · 7153330

Rapid, modified oxidase test for oxidase-variable bacterial isolates.

J J Tarrand·D H Gröschel
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Abstract

A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive). The bacterial inoculum was obtained with a cotton-tipped swab instead of a loop or wooden applicator, and the reaction required less than 15 s.

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BacteriaDimethyl SulfoxideIndicators and ReagentsOxidoreductasesTetramethylphenylenediamineJournal Article

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J J Tarrand, D H Gröschel. 1982. Rapid, modified oxidase test for oxidase-variable bacterial isolates.. https://doi.org/10.1128/jcm.16.4.772-774.1982

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