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Nerve growth factor: subunit interactions in the mouse submaxillary gland 7S complex.

R E Silverman·R A Bradshaw
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Abstract

Sedimentation velocity analyses of the isolated subunits of mouse 7S nerve growth factor (NGF) and their binary complexes have been performed. The 2.5S (beta) form of NGF binds 2 alpha subunits independent of the presence of gamma subunits. Two gamma subunits are also complexed by 2.5S NGF; however, this interaction is dependent on the presence of a carboxyl terminal arginine residue on the beta polypeptide chain. The partial loss of this residue in 2.5S NGF preparations results in a ternary complex(es) with a reduced sedimentation coefficient(s). The two binary complexes formed (alpha 2-beta and beta-gamma 2) show markedly different pH stability profiles. The alpha and gamma subunits do not form a demonstrable complex, but apparently can interact in the ternary complex to render it more stable than the sum of the alpha-beta and beta-gamma interactions.

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AnimalsChemical PhenomenaChemistryChromatography, GelHydrogen-Ion ConcentrationMaleMiceMice, Inbred ICRNerve Growth FactorsPeptidesSubmandibular GlandUltracentrifugationComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R E Silverman, R A Bradshaw. 1982. Nerve growth factor: subunit interactions in the mouse submaxillary gland 7S complex.. https://doi.org/10.1002/jnr.490080204

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