PubMed · 7157085
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P E Peters. 1982. [Lymphography--an obsolete method?].. https://pubmed.ncbi.nlm.nih.gov/7157085/
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Consensus degenerate primers specific for the L1 and E1 regions were used to amplify anogenital HPV-DNA fragments. The mixture of the viral fragments was then directly sequenced with HPV-6, -11, -16, -18 and -33 type-specific computer-designed oligonucleotides as sequencing primers. The linking of a consensus primer-generated PCR amplification with type-specific primer-mediated direct sequencing and computer data bank analysis provided precise, more objective viral detection in the simultaneous presence of different HPV types. The method was successfully adapted for viral typing in clinical lesions which simultaneously contained different anogenital HPV sequences.
The efficacy of transmission block technique with contiguous photon irradiation of inguinal as well as pelvic and perineal structures has been reported in literature. In this paper, a simple and accurate method for the proper fabrication of transmission block has been described and also demonstrated to be effective and useful. The procedures for a precise geometrical alignment and dose verification are also detailed.
A patient with a seminoma, squamous cell carcinomas of the skin and anus, Bowen's disease and lymphopenia is reported. The underlying aetiology is thought to be impaired cell mediated immunity and susceptibility to viral infections.