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Abnormal embryonic development induced by antiserum: a rapid method to isolate the responsible galactoprotein antigens.

C C Leung
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Abstract

It has been well established that heterologous antiserum against whole rat kidney homogenate when injected into pregnant rats during the organogenetic period may induce abnormal embryonic development. A one-step rapid method has been developed to isolate the responsible rat renal antigens by lectin-affinity chromatography using soy bean agglutinin (SBA) or ricinus communis agglutinin (RCA120). The purified antigens appeared to be galactoproteins with terminal nonreducing D-galactose and/or N-acetyl-D-galactosamine. Rabbit antisera produced against the galactoproteins were potent teratogenic agents.

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AnimalsAntigen-Antibody ComplexAntigensChromatography, AffinityEmbryo, MammalianFemaleGlycoproteinsImmune SeraPregnancyRatsRats, Inbred StrainsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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C C Leung. 1982-11-20. Abnormal embryonic development induced by antiserum: a rapid method to isolate the responsible galactoprotein antigens.. https://doi.org/10.1002/jez.1402240114

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