PubMed · 7179476
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I Ia Barskiĭ, V A Danilov, Iu E Korovianskiĭ, A I Mazurov, Iu M Rozanov. 1982. [Television fluorescence microscope].. https://pubmed.ncbi.nlm.nih.gov/7179476/
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A group supervision project in psychiatry in general practice was established in 1992. The aims were to increase competence and interest in psychiatry and to contribute to a supervision model in psychiatry among general practitioners in a sparsely populated area. The group leader, who was a psychiatrist with competence in group analysis met with seven physicians for a three hour evening meeting once a month over a period of two years. The meetings were structured with a planned session at the beginning followed by a more open session. A focus group approach was used as the evaluation method. This method showed its usefulness in enabling the group to adjust its structure after an evaluation conducted after one year had passed and the conclusion at the end of the project period was: Group supervision can contribute to increased competence in psychiatry among general practitioners. It seems to increase the doctors' confidence and endurance in relation to psychiatric problems in family practice and provides an important opportunity to share difficult experiences with colleagues.
OBJECTIVE: To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS: The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to hydrolysis of phosphotyrosine. The time-resolved immunofluorometric assay employs tyrosine-phosphorylated substrates, immobilized on microtitre wells. After incubation with PTP, the remaining phosphotyrosine residues are reacted with an antiphosphotyrosine antibody. The immunocomplexes formed are detected with an alkaline phosphatase (ALP)-labeled second antibody. The phosphate ester of 5' fluorosalicylate (FSAP) is used as substrate. The fluorosalicylate produced forms highly fluorescent complexes with Tb3+ - EDTA in alkaline solution. The fluorescence is measured with a time-resolved fluorometer. RESULTS: The truncated form of the T-cell protein tyrosine phosphatase (TCdeltaC11 PTP) was determined in the range 1100-36,500 U/L by the fluorometric assay and 36-7100 U/L by the time-resolved immunofluorometric assay. CONCLUSIONS: The two nonisotopic assays should prove beneficial for the determination and study of various PTP.