Nuclear chromatin changes during post-natal myocardial development.

C J Limas; C Chan-Stier

doi:10.1016/0005-2787(78)90280-0

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Nuclear chromatin changes during post-natal myocardial development.

C J Limas·C Chan-Stier

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Abstract

The proliferative capacity of rat myocardium declines rapidly during the first few weeks of post-natal life. In order to gain insight into the mechanisms involved in this decline, we studied the structure and function of nuclear chromatin from isolated rat myocardial cells during post-natal growth. Chromatin template activity decreased progressively (7.5 +/- 0.3 pmol [3H]UTP/microgram DNA per min at age 5 days compared to 2.2 +/- 0.1 pmol [3H]UTP/microgram DNA per min at age 6 months) and was associated with a decrease in the number of transcription initiation sites. This decline was accompanied by changes in chromatin structure as evidenced by: (a) decreased susceptibility to DNAase I digestion with advancing age, (b) decreased poly-L-lysine binding (60% decrease between day 5 and six months of age), (c) progressive decline in positive ellipticity of circular dichroism spectra between 250--300 nm, and (d) derivative melting profiles showing a decrease in DNA regions bound by non-histone proteins and concomitant increase in histone-bound regions. The protein composition of myocardial chromatin also changed during post-natal development, chiefly due to a progressive increase in the histone/DNA ratio. These results indicate substantial changes in the organization and functional capacity of myocardial chromatin during early post-natal growth. These changes accompany, and may contribute to, the restriction in the proliferative capacity of myocardial cells.

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Aging Animals Cell Nucleus Chromatin Circular Dichroism DNA Heart Histones In Vitro Techniques Kinetics Myocardium RNA Rats Templates, Genetic Transcription, Genetic Journal Article

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C J Limas, C Chan-Stier. 1978-11-21. Nuclear chromatin changes during post-natal myocardial development.. https://doi.org/10.1016/0005-2787(78)90280-0

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