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PubMed · 7200984

Crystallization and preliminary X-ray data for chloroperoxidase.

B Rubin·J VanMiddlesworth·K Thomas·L Hager
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Abstract

The enzyme chloroperoxidase from the fungus Caldariomyces fumago has been crystallized in a form suitable for detailed three-dimensional x-ray structure determination. Crystals grown from polyethylene glycol solutions display high resolution diffraction patterns indicating the orthorhombic space group C2221: a = 151.1 (3), b = 57.9 (4), c = 102.7 (9) A, V = 9 X 10(5) A3. A volume to mass ratio Vm = 2.8 A3/dalton for 8 molecules in the unit cell and a measured density of 1.35 g/cm3 suggest that the asymmetric unit contains a single chloroperoxidase molecule.

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AnimalsChloride PeroxidaseCrystallizationMitosporic FungiPeroxidasesProtein ConformationX-Ray DiffractionJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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B Rubin, J VanMiddlesworth, K Thomas, L Hager. 1982-07-10. Crystallization and preliminary X-ray data for chloroperoxidase.. https://pubmed.ncbi.nlm.nih.gov/7200984/

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