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Application of quantitative high-performance thin-layer chromatography in the antibiotic industry.

F Kreuzig
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Abstract

For the in-process control of antibiotic fermentations and for routine assays of samples for scaling up and for pure products, quantitative high-performance thin-layer chromatography (HPTLC) can be used with advantage. Rapid chromatography on high-performance layers, combined with an automatic spraying device for exact derivatization on the plate and precise computation of the calibration line within an automatic measurement and evaluation, represents a new, inexpensive analysis system. There are only 2 min of labour time (one fifth of that required in thin-layer chromatography) required for one sample and the total analysis time varies from 3 to 9 min (one third to one quarter of that required in thin-layer chromatography) based on one plate with 12 samples. The 95% confidence limits (N = 10) range between 0.5 and 3.0%.

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Anti-Bacterial AgentsChromatography, High Pressure LiquidChromatography, Thin LayerDrug IndustryFermentationGramicidinMethodsJournal Article

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F Kreuzig. 1977-11-11. Application of quantitative high-performance thin-layer chromatography in the antibiotic industry.. https://doi.org/10.1016/s0021-9673(01)92057-6

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