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PubMed · 7211109

Rat embryo development in vitro.

S K Roy·J Sengupta·S K Manchanda
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Abstract

We report the first successful culture of 8-cell/morula stage rat embryos in a fully synthetic medium supplemented with 3% crystalline bovine serum albumin. Eighty-four per cent of morulae developed to blastocysts, showing that this is a highly efficient culture medium for in vitro studies on rat pre-implantation embryos. Blastocyst formation was severely inhibited by antioestrogen (nafoxidine 3 microgram/ml) but no further reversal was obtained by giving oestrogen to culture medium containing this antagonist.

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AnimalsBlastocystCleavage Stage, OvumCulture MediaEstradiolFemaleMorulaNafoxidinePregnancyRatsJournal ArticleResearch Support, Non-U.S. Gov't

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S K Roy, J Sengupta, S K Manchanda. 1981. Rat embryo development in vitro.. https://doi.org/10.1530/acta.0.0960546

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