Mouse glomerular culture.

J S Hunt; A E Jackson; W A Day; A R McGiven

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PubMed · 7225290

Mouse glomerular culture.

J S Hunt·A E Jackson·W A Day·A R McGiven

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Abstract

A method is described for the culture of isolated intact mouse glomeruli. Cultures showed adherent glomeruli after 2 days and from 2-4 days a monolayer of cells began to develop around the points of adherence. The predominant cell (Type I) was 80-200 micrometer in diameter with cytoplasmic extensions, later becoming polyhedral and often multinucleated. Type II spindle cells, 70-100 micrometer in length, were less prominent in the earlier stages and later formed sheaves between Type I cells. Small phagocytic Type III cells, 10-20 micrometer in diameter, were not prominent in cultures from normal kidneys and tended to form small clusters on top of the Type I cell monolayer.

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Animals Cell Movement Culture Techniques Kidney Glomerulus Mice Mice, Inbred Strains Microscopy, Electron Phagocytosis Time Factors Journal Article Research Support, Non-U.S. Gov't

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J S Hunt, A E Jackson, W A Day, A R McGiven. 1981. Mouse glomerular culture.. https://pubmed.ncbi.nlm.nih.gov/7225290/

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