A simple method for in vitro studies with rodent urinary bladders.

C D Jackson; G Newport

doi:10.1007/bf02618066

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A simple method for in vitro studies with rodent urinary bladders.

C D Jackson·G Newport

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Abstract

A method was developed for the in vitro study of rodent urinary bladders. The method consists of everting and distending the urinary bladder in a manner to allow exposure of the luminal surface of the urothelium during in vitro incubation while maintaining the integrity of the structure and morphology of the bladder. A technique for selectively removing the urothelium with SDS buffer for biochemical analysis was described. Incorporation of [3H]leucine into urothelial protein was linear over a 4 h period in the presence of tissue culture medium, but no significant incorporation occurred when urine was used as incubation medium. Autoradiography indicated the [3H]leucine incorporation was almost exclusively in the urothelial cells with essentially no incorporation by cells below the tunica propria.

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Animals Epithelium Leucine Male Mice Mice, Inbred BALB C Organ Culture Techniques Sodium Dodecyl Sulfate Urinary Bladder Journal Article

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C D Jackson, G Newport. 1981. A simple method for in vitro studies with rodent urinary bladders.. https://doi.org/10.1007/bf02618066

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