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A stable, sensitive assay for human fibronectin.

H D Todd-Kulikowski·R G Parsons
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Abstract

A solid-phase radioimmunoassay system to quantitate human fibronectin has been developed. This assay utilizes 125I-labeled affinity purified IgG directed against human plasma fibronectin. The sensitivity of this system is comparable to conventional (labeled antigen) radioimmunoassays having a detection limit of approximately 0.5 ng. This assay is relatively rapid (less than 24 h), the reagents are stable at 4 degrees C (less than 2 months), and the reproducibility is excellent. Both human plasma and cellular fibronectin react equivalently in this assay.

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AnimalsFibronectinsHumansImmunoglobulin GMethodsPreservation, BiologicalRabbitsRadioimmunoassayJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

H D Todd-Kulikowski, R G Parsons. 1981. A stable, sensitive assay for human fibronectin.. https://doi.org/10.1016/0022-1759(81)90051-x

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