Halothane modifies colchicine binding to tubulin.

G A Vergara; A Livingston

doi:10.1159/000137559

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Halothane modifies colchicine binding to tubulin.

G A Vergara·A Livingston

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Abstract

Tubulin extracted from rat brains was incubated at 37 degrees C with 3H-colchicine in the presence and absence of 28 mM halothane. The amount of 3H-colchicine bound to tubulin was reduced by nearly 50% at a colchicine concentration of 5 x 10(-5) M when halothane was present. At low (1 x 10(-7) M) and at high (1 x 10(-3) M) concentrations of colchicine, halothane did not affect the binding as much. The inhibition of colchicine-binding to tubulin by halothane appeared to be non-competitive.

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Animals Brain Chromatography, Gel Colchicine Halothane Kinetics Protein Binding Rats Rats, Inbred Strains Tubulin Journal Article Research Support, Non-U.S. Gov't

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G A Vergara, A Livingston. 1981. Halothane modifies colchicine binding to tubulin.. https://doi.org/10.1159/000137559

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