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PubMed · 735641

A sequential extraction method for RNA from rabbit liver.

B Axelsson·A Norgren·L Holmquist·A Deutsch
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Abstract

When naphthalene-1,5-disulfonate (NDS) and phenol-cresol were used for extraction of rRNA from rabbit liver approximately 80% of the extractable RNA was recovered. By subsequent treatment of the resulting insoluble interface with 4-aminosalicylate and triisopropylnaphthalenesulfonate (PAS-TIPNS) the remaining RNA was extracted. Subcellular fractionation and polyacrylamide gel electrophoresis or sucrose density gradient analysis showed that this last fraction contrained 28s RNA from membrane-bound polysomes. Applying the sequential extraction method for isolation of poly(A)-containing mRNA showed that only a minor part of that RNA was extracted with NDS while the rest was recovered by PAS-TIPNS treatment of the interface. Thus, a purification of poly(A)-containing mRNA was possible when the sequential extraction method was used.

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Aminosalicylic AcidsAnimalsCentrifugation, Density GradientChromatography, GelElectrophoresis, Polyacrylamide GelLiverMicrosomes, LiverNaphthalenesulfonatesPolyribosomesRNARNA, RibosomalRabbitsSubcellular FractionsJournal Article

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B Axelsson, A Norgren, L Holmquist, A Deutsch. 1978. A sequential extraction method for RNA from rabbit liver.. https://doi.org/10.3891/acta.chem.scand.32b-0671

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