A murine C4 molecule with reduced hemolytic efficiency.

J P Atkinson; K McGinnis; L Brown; J Peterein; D Shreffler

doi:10.1084/jem.151.2.492

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A murine C4 molecule with reduced hemolytic efficiency.

J P Atkinson·K McGinnis·L Brown·J Peterein·D Shreffler

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Abstract

C4 functional activity and antigenic levels were determined in H-2-congenic mouse strains. In strains with the H-2w7 haplotype, the C4 hemolytic activity per unit of residual Ss antigenic activity, after depletion of the nonfunctional Slp-positive molecules was 25-33% that found with other H-2 haplotypes. This reduced hemolytic efficiency was not the result of either a more labile C4 molecule or of the presence of inhibitors. Moreover, other strains with comparable antigenic concentrations of Ss (C4) and Slp has three- to fourfold higher levels of C4 hemolytic activity. Based on these data and previously reported structural differences between C4 molecules from the H-2w7 haplotype compared with other standard H-2 haplotypes, the reduced hemolytic efficiency of this molecule is probably secondary to alterations in the structure of its alpha-chain.

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Animals Antigens Blood Proteins Complement C4 H-2 Antigens Hemolysis Male Mice Mice, Inbred Strains Protein Conformation Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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J P Atkinson, K McGinnis, L Brown, J Peterein, D Shreffler. 1980-02-01. A murine C4 molecule with reduced hemolytic efficiency.. https://doi.org/10.1084/jem.151.2.492

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