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PubMed · 7357019

Evidence for active phospholipase A in mouse plasma.

M P Gillett·E M Costa·J S Owen
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Abstract

Mouse, rat and human plasma were exposed to minimum concentrations of disulphide or minimum pre-incubation at 55 degrees C in order to inhibit lecithin : cholesterol acyltransferase activity completely. The plasma samples were subsequently incubated at 37 degrees C and changes in individual phospholipid concentrations determined. Significant utilization of phosphatidylcholine and formation of lysophosphatidylcholine occurred only in disulphide-treated mouse plasma and this was accompanied by a decrease in total phospholipid concentration. When disulphide-treated mouse plasma was incubated with [U-14C]phosphatidylcholine radioactivity was additionally recovered in the lysophosphatidylcholine, non-esterified fatty acid and glycero-3-phosphocholine fractions; maximum conversion occurred at close to physiological pH. These observations suggest that phospholipase A and lysophosphatidylcholine hydrolase enzymes are active in mouse plasma but that phospholipase A is either absent or inactive in rat and human plasma.

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AnimalsCholesterolDithionitrobenzoic AcidHydrogen-Ion ConcentrationLysophosphatidylcholinesMaleMicePhosphatidylcholine-Sterol O-AcyltransferasePhosphatidylcholinesPhospholipasesPhospholipases ARatsJournal Article

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M P Gillett, E M Costa, J S Owen. 1980-02-22. Evidence for active phospholipase A in mouse plasma.. https://doi.org/10.1016/0005-2760(80)90166-6

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