PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 737200

Determination of elastolytic activity using a conductimetric method.

H Bakala·J Wallach·M Hanss
View original source ↗Export metadata ↓

Abstract

The source did not provide an abstract. Follow the original record for more information.

Explore related subjects

ConductometryElastinKineticsPancreatic ElastaseSubstrate SpecificityJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

H Bakala, J Wallach, M Hanss. 1978. Determination of elastolytic activity using a conductimetric method.. https://doi.org/10.1016/s0300-9084(79)80357-0

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

EOF measurement by detection of a sampling zone with end-channel amperometry in microchip CE.

A simple method for EOF measurement by detection of sampling zones with end-channel amperometry in microchip CE is developed. This method is based on the principle of the Kohlrausch regulating function (KRF). A dilute electroactive ionic species is added to the BGE as a continuously eluting electrophore which is used as a probe. When a BGE-like sample at a different concentration is injected, a peak of sampling zone appears and the migration time is related to EOF. In a microchip CE with hybrid PDMS/glass channel, a cathodic EOF of the hybrid glass/PDMS microchip was measured by end-channel amperometry; the effects of sample concentration and different probes on EOF rate were discussed. The present method was applied to monitor EOF rates in glass and in PDMS microchips. There was no significant difference between the values of EOF rates measured by the present method and the current-monitoring method. Detection of nonelectroactive analytes K(+), Na(+), and Li(+) can also be accomplished by the indirect amperometric method. Hence, the effective mobility of analyte can be accurately obtained.

Conductometry↗
PubMed2006

Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector - CE microchips.

This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off-channel electrodes are deposited around side channels by Au sputtering and patterned using a standard "lift-off" process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab-on-a-chip concept.

Conductometry↗
PubMed2005

[Capacitively coupled contactless conductivity detection in capillary electrophoresis].

Capacitively coupled contactless conductivity detection (C4D) is a new detection technique has been developed in recent years and used mainly in capillary electrophoresis and microchip electrophoresis. The characteristics of C4D detector are simple in structure, easy in miniaturization and integration, and free of electrodes contamination, which are common problems in an electrochemical detection. The principles and progresses of C4D in capillary electrophoresis are reviewed.

Conductometry↗