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PubMed · 7389110

17-Hydroxycorticosteroids in urine.

M Simon·M Babich-Armstrong
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Abstract

A new method has been devised to extract 17-hydroxycorticosteroids (17OH-CS) from a hydrolyzed urine using an EXTUBE fiber column. This extraction was combined with a modified Porter-Silber color reaction to quantitate total urinary 17OH-CS. The new method takes only half the time required in older methods. Recovery of tetrahydrocortisone in the new method was improved to 85--95% compared with 70% in the previous method. Between-run precision (C.V.%) was concentration dependent in both methods and was improved to 5--7% compared with 10--18% in the previous method. Evidence was obtained to demonstrate that the new methodology would provide the same information and interpretation as the older, more cumbersome method.

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BibTeXRIS

M Simon, M Babich-Armstrong. 1980-04-11. 17-Hydroxycorticosteroids in urine.. https://doi.org/10.1016/0009-8981(80)90235-1

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PubMed2006

Comprehensive study of urinary cortisol metabolites in hyperthyroid and hypothyroid patients.

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